Detection range: contact us (or send an email to biolzy@yahoo.cn) Detection of the target: human / animal Sample forms of Tetanus ELISA Kit : serum / plasma / cell culture supernatant / other biological fluids (specifically, please contact) Preservation and transport of 4 ℃ /: Short-term and long-term stored at -20 ℃ Range of applications: research with the detection reagent When one is doing ELISA test, he must note the following things: A formal test, should be compared to positive control and negative control test conditions, the sample to be assayed in duplicate should be made, in order to ensure the accuracy of the experimental results. Sometimes the background, which shows that non-specific reactions can be used sheep serum, rabbit serum or BSA closed.
Standards or samples are then added to the purified antibody coated microtiter plate, made of solid phase carrier. Tetanus ELISA Kit is bound by the anti-Tetanus Ab, antibody microporous, biotinylated anti-Tetanus Ab, antibody, HRP-labeled avidin, After thorough washing, TMB color substrate. TMB peroxidase catalyzed into the blue, and acid into the final yellow. The depth of color and Tetanus Ab, samples were positively correlated. Measured absorbance in the 450nm wavelength with a microplate reader (OD), calculate the sample concentration.Read More: buy Tetanus ELISA Kit
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