Tumor Necrosis Factor-alpha (TNF-alpha) is a 17.5kDa, 157 amino acid protien that is a potent lymphoid factor, which enerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF alpha has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. TNF alpha the primary mediator of immune regulation. The biosynthesis of TNF alpha is tightly controlled being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.Phoenix Pharmaceutical’s Human TNF-α ELISA Kit is designed to measure the concentra-tion of Human TNF-α from Human serum/plasma, or conditioned medium.
The immunoplate in this kit is pre-coated with Anti-Human TNF-α Capture Antibody and the non-specifc binding sites are blocked. The Human TNF-α in the sample or in the standard solution can bind to the capture antibody immobilized in the wells. After washing procedure, the biotinylated anti-Human TNF-α Detection Antibody which can bind to the Human TNF-α trapped in the wells is added. The enzyme-substrate reaction is terminated by the addition of a stop solution. The intensity of the color is directly proportional to the amount of Human TNF-α in the standard solutions or samples. A standard curve of Human TNF-α with known concentration can be established accordingly. The Human TNF-α with unknown concentration in samples can be determined by extrapolation to this standard curve.
No comments:
Post a Comment