Because cycloheximide has a very strong toxicity, including damage to DNA, leading to fetal malformations and other effects on reproduction (including birth disorders and sperm toxicity), it is generally only used in vitro research applications, Actidioneis not appropriate use of antibiotics in the human body as a. In the past Actidione was used in agriculture as a fungicide, but because of its increasing awareness of the dangers of this usage has been very small. Alkali can destroy cycloheximide, so if the work surface or container contaminated by cycloheximide harmless as long as the use of alkaline solution (such as soap) to wash it. In vitro applications in molecular biology cycloheximide can be used to determine the protein (enzyme) half-life. Using cycloheximide-treated cells, and then use the western blot (Western blot) experiments to show the protein to be studied over time, the number of changes. Because cycloheximide hinder protein synthesis, decreasing protein within the cell.
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