Monday, June 4, 2012

Detailed Knowledge About SCGF-a ELISA Kit


A method for producing a hematopoietic stem cell growth factor (SCGF) polypeptide having erythroid burst-promoting activity and granulocyte macrophage colony-promoting activity on bone marrow cells of a mammal, comprising culturing the transformant of claim 26 in a medium that promotes the production and accumulation of the polypeptide and recovering the polypeptide from the resultant culture.

Item name: SCGF-a ELISA Kit
Specification: 96Test

 SCGF-a ELISA Kit The present invention relates to a novel hematopoietic growth factor protein termed hematopoietic stem cell growth factor (hereinafter referred to as “SCGF”) which acts on hematopoietic stem cells to maintain their survival and to induce their proliferation and differentiation. The present invention also relates to a gene coding for SCGF; a vector comprising the gene; a transformant transformed with the vector; a method for producing SCGF; and a method for separating and purifying SCGF. The present invention further relates to the use of SCGF as a therapeutic for hematopoietic insufficiency derived from irradiation or chemotherapy for patients with various hematopoietic diseases or cancers; or the use of SCGF as a reagent for diagnostic analysis. The present invention also relates to the use of SCGF in bone marrow transplantation for the purpose of hematopoietic recovery, for which hematopoietic stem cells can be amplified with SCGF in vitro in a small amount of bone marrow aspirates; and the use of SCGF in gene therapy to improve the efficiency of a gene transfer into hematopoietic stem cells. The invention also relates to a vector developed to isolate the SCGF gene, as well as a method for isolating the gene. The vector and the method can provide promising tools to search for other novel genes for unknown proteins.
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