Gastric inhibitory polypeptide (GIP), also known as the glucose-dependent insulinotropic peptide is a member of the secretin family of hormones. GIP, along with glucagon-like peptide-1 (GLP-1), belongs to a class of molecules referred to as incretins.
GIP ELISA Kit allows for the specific measurement of mouse GIP (glucose-dependent insulintropic polypeptide) concentrations in cell culture supernates, serum, and plasma. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GIP present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for GIP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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