Rat C-peptide is a polypeptide molecule comprising 31 amino acid residues with molecular mass of about 3.2 kDa. C-peptide is originated from proinsulin, which is synthesized in the β-cells of the Islets of Langerhans and cleaved enzymatically releasing insulin and C-peptide. Proinsulin is cleaved by two Ca-dependent en-dopeptidases (prohormone convertases) PC2 and PC3. Endopeptidase PC2 (type II) cleaves at the A/C chain junction of proinsulin (between amino acid residues 65 and 66) and PC3.
Rat C-Peptide Elisa kit is for research use only. Do not administer it to human and it should be kept at 2-10 degrees C. On the surface of the microplate wells, goat anti rabbit IgG is coated and when sample is reacted with rabbit and InsC-Peptide and biotinylated rat InsC-peptide in the well, the goat antibody captures the complex of the rabbit antibody and rat Ins C-peptide formed in competitive reaction. Subsequentreaction with horseradish peroxides (HRP)-conjugated Streptzvidin to the complex on the well surface results in labeling of the complex with HRP which generates the signal for the presence of rat InsC-peptide in the sample.
Read More: buy Rat C-Peptide Elisa kit
No comments:
Post a Comment