The Principle of T-NOS ELISA Kit

Item name:t-NOS ELISA Kit

Specification: 96Test

T-NOS ELISA kit uses double antibody sandwich method determination of the level of the specimens of human nitric oxide synthase (NOS). With purified nitric oxide synthase enzyme (NOS) antibody coated microtiter plate made of solid phase antibody, followed by adding nitric oxide synthase (NOS), to the microporous coated monoclonal antibody with an HRP-labeled nitric oxide synthase (NOS) antibody binding, the formation of antibody - antigen - enzyme-antibody complex after a thorough washing, TMB substrate added color. TMB in HRP enzyme-catalyzed conversion into the blue, and acid into the final yellow. The depth of color and the sample in nitric oxide synthase (NOS) was positively correlated. Measured absorbance in the 450nm wavelength with a microplate reader (OD), calculated by standard curve samples of human nitric oxide synthase (NOS) concentration.

Nitrous oxide, commonly known as laughing gas or sweet air, is a chemical compound with the formula N2O. It is an oxide of nitrogen. At room temperature, it is a colorless non-flammable gas, with a slightly sweet odor and taste. It is used in surgery and dentistry for its anesthetic and analgesic effects. It is known as "laughing gas" due to the euphoric effects of inhaling it, a property that has led to its recreational use as a dissociative anesthetic. It is also used as an oxidizer in rocketry and in motor racing to increase the power output of engines. At elevated temperatures, nitrous oxide is a powerful oxidizer similar to molecular oxygen.

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