Some Knowledge About TSI ELISA Kit

Item name:TSI ELISA  KitSpecification:96Test

Sample forms: serum / plasma / cell culture supernatant / other biological fluids (specifically, please contact)
Preservation and transport: short-term 4 ° C
Range of applications: research with the detection reagent

A thyroid stimulating immunoglobulin, or TSI, is a type of molecule created by the immune system, known as an autoantibody.

Human thyroid stimulating immunoglobulin (TSI) ELISA Kit Liquid samples:
Including serum, plasma, urine, pleural effusion, cerebrospinal fluid, cell culture supernatant.
Serum: After coagulation at room temperature for 10-20 minutes, centrifuged for 20 minutes or so (2000-3000 r / min). Carefully collect the supernatant. If precipitation in the preservation process, Centrifugal again.
Plasma should be based on the requirements of the specimen: EDTA, sodium citrate or heparin as an anticoagulant, 10-20 minutes after mixing, centrifuged for 20 minutes or so (2000-3000 r / min). Carefully collect the supernatant. If precipitation in the preservation process, Centrifugal again.
Urine: collect a sterile tube. Centrifuged for 20 minutes or so (2000-3000 r / min). Carefully collect the supernatant. If precipitation in the preservation process, Centrifugal again. Pleural and peritoneal effusions, cerebrospinal fluid with reference to this practice.
The cell culture supernatant: the detection of secretory component, collected with sterile tube. Centrifuged for 20 minutes or so (2000-3000 r / min). Carefully collect the supernatant.
Detection of intracellular components, with PBS (PH7.2-7.4), diluted cell suspension, the cell concentration reached about 1 million / ml. By repeated freezing and thawing, cell damage and release of intracellular components. Centrifuged for 20 minutes or so (2000-3000 r / min). Carefully collect the supernatant. If precipitation in the preservation process, Centrifugal again.
Tissue samples: After cutting samples, check the weight. Adding a certain amount of PBS, pH 7.4 and Rapidly frozen with liquid nitrogen and stored. The specimens remained 2-8 ° C melt temperature. By adding a certain amount of PBS (pH 7.4 and) specimen homogenate by hand or homogenizer. Centrifuged for 20 minutes or so (2000-3000 r / min). Carefully collect the supernatant. Be detected after a repackaging, spare the rest frozen.

Read More: TSI ELISA Kit suppliers