Wednesday, May 30, 2012

Some Knowledge of 3X Flag Peptide

 3X Flag Peptide

Item name: 3X Flag Peptide
Molecular Formula: C120H169N31O49S1
Molecular Weight: 2861.87g/mol
Description: This product is lyophilized powder. 3X Flag Peptide is used in competitive elution of 3X FLAG fusion proteins from the ANTI-FLAG M2 monoclonal antibody in solution or bound to agarose on the ANTI-FLAG M2 agarose affinity gel. 3X Flag Peptide is also used for laboratory use only but not for drug, household or other uses. This product shoud be sealed between 2-8°C.

The 3xFLAG system is an improvement upon the original system by fusing 3 tandem FLAG® epitopes for a total of 22 amino acids (Figure 1). Detection of fusion proteins containing 3xFLAG is enhanced up to 200 times more than any other system. Like the original FLAG tag, 3xFLAG is hydrophilic, contains an Enterokinase cleavage site, and is relatively small. Therefore, the risk of altering protein function, blocking other epitopes or decreasing solubility is minimized. In cases of low-level expression, 3xFLAG is ideal. Our selection of CMV vectors provides options for transient or stable expression of 3xFLAG fusion proteins.
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