Thursday, May 31, 2012

General Information About sc-KIT/sCD117 ELISA Kit


Item name: sc-KIT/sCD117 ELIS Kit
Specification :96Test

People can buy the best cost-effective sc-KIT/sCD117 ELIS Kit in Master Germany Co., Ltd. production. Welcome to buy sc-KIT/sCD117 ELIS Kit as long as sc-KIT/sCD117 ELIS Kit is your best choice. Welcome to contact the company anytime.

Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.

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